腸道菌增菌肉湯

成分(g/L)
Pancreatic Digest of Gelatin/
胰酶 明膠消化物             10.0
Glucose monohydrate /
葡萄糖                       5.0
Disodium hydrogen phosphate dihydrate/
磷酸氫二鉀                   8.0
Potassium dihydrogen phosphate/
磷酸二氫鈉                   2.0
Dehydrated Ox Bile/
脫水牛膽粉                  20.0
Brilliant Green/煌綠           0.015
pH值7.2 ± 0.2             25℃
用法
Suspend 45.0g / liter,and heat to boiling for 1  to  2 minutes. Transfer 120-ml  portions to  250-ml  volumetric flasks or 9-ml portions to test tubes, all being capped with cotton plugs or loose-fitting caps. Heat at 100 °C for 30 min and cool immediately.
稱取本品 45.0g,加熱攪拌溶解于 1000ml 蒸餾水中,分裝三角瓶每瓶 120ml或分裝試管 ,每管 9ml,100℃水浴加熱 30 分鐘 , 迅速在流動的冷水中冷卻,無需高壓滅菌。